This
PX-JX2 device
entails the unusual PX state of DNA.
We found that it was not possible to bind RNA strands to this state, so
we were unable to control this device.
To overcome this problem, we developed a 'cover strand' strategy,
wherein we made a single strand of DNA to contain the control regions that
produce both states of the device.
We then used RNA strands to cover up the one we did not want. The machine cycle of the device then
looks as follows:
This
system works perfectly well to produce the states needed for this device. The device should now be available to
respond to RNA produced by transcriptional circuitry.
Return to Ned Seeman's
Nanotechnology Page
Return
to Homepage for
Ned Seeman's Lab